The absorbance values for all of your samples need to be between 0.1 and 1.0 absorbance units for best results at all wavelengths of interest. UV-VIS SPECTROMETERS (Go Direct UV-VIS, Go Direct UV-VIS/Fluorescence, etc)ġ. Detailed instructions are available here: How do I check the lamp output of my spectrometer? If Uncalibrated mode shows flat areas, this indicates that something may be wrong with the light source. To test this, switch the spectrometer to Uncalibrated mode and look at the spectrum across the entire wavelength range. The light source in the spectrometer may have burned out or is otherwise not working properly. Why is the absorbance reading on my device (spectrometer/colorimeter) unstable or nonlinear at values above 1.0?Ģ.
If this is the case, then start by diluting your samples. If your samples are too concentrated, they will cause too much noise and result in empty cells or readings of 3. VISIBLE ONLY SPECTROMETERS (SpectroVis Plus, Go Direct SpectroVis Plus, Go Direct Visible, etc)ġ. Review the tips below for your specific spectrometer type.
LOGGER PRO SPECTROMETER ERROR SOFTWARE
When this happens, the software may report a blank cell or a value above 3. This can happen either when the light source is weak in that spectral region, or when the sample is so strongly absorbing that very little light strikes the detector.
Sometimes the counts from the light striking a pixel is not sufficiently different from the counts when the pixel is in the dark to make a reliable absorbance reading.
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